Pathological changes in the preparation of bone-skin flap with allogeneic bone induction

doi:10.3969/j.issn.2095-4344.2013.44.013

Abstract

Abstract:

BACKGROUND: Autologous bone-skin flap transplantation is the best method for the repair of composite tissue defect, but the repair ability is limited, with big trauma, new tissue defect and a certain dysfunction. Allogeneic bone has the osteoinductive capacity, which can be used to prepare the bone-skin flap.
OBJECTIVE: To research the pathological change of the allogenic bone during the prefabrication of bone-skin flap with allogeneic bone implant.
METHODS: The experimental animals were Bama miniature pigs. Deep-frozen allogenic bone was implanted in iliac artery-supported tissue flap compartment of miniature pigs. After operation, the local reactions were observed, the allogenic bone were studied by general observation and histological analysis at 4, 8, 12 and
16 weeks after implantation respectively.
RESULTS AND CONCLUSION: Obvious inflammation reaction was not observed in the surgical zone. The allogeneic bone was vascularized at 4 weeks after implanted into the flap tissue without obvious osteoblast-like cells. The vascularization, bone resorption and uneven distributed osteoblast-like cells and osteoclast-like cells were observed at 8 weeks after implantation, and new bone formation was observed. At 12 weeks after implantation, new bone formation and bone absorption was strengthened, and the morphology of the bone graft was changed. At 16 weeks after implantation, allogenic bone turned into fragments and absorbed, and no new bone formation was observed. The results indicated that during the prefabrication of bone-skin flap with allogeneic bone implantation, the pathological changes of the allogeneic bone was observed with time prolonging, and the bone-skin flap should be transplanted in time.

Key words: tissue transplantation, surgical flaps, stromal cells, cryopreservation, osteoclasts

CLC Number:

R318

Han Qing-luan, Li Wei, Fan Hong-jin, Zhang Bo, Li Zheng-xun. Pathological changes in the preparation of bone-skin flap with allogeneic bone induction[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(44): 7733-7738.

Figures/Tables 3

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